Methanol oxidation under physiological conditions using methanol dehydrogenase and a factor isolated fromHyphomicrobiumX
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چکیده
منابع مشابه
Microbial oxidation of methane and methanol: crystallization of methanol dehydrogenase and properties of holo- and apomethanol dehydrogenase from Methylomonas methanica.
Procedures are described for the purification and crystallization of methanol dehydrogenase from the soluble fraction of the type I obligate methylotroph Methylomonas methanica strain S1. The crystallized enzyme is homogeneous as judged by acrylamide gel electrophoresis and ultracentrifugation. The enzyme had a high pH optimum (9.5) and required ammonium salt as an activator. In the presence of...
متن کاملIn silico studies of the mechanism of methanol oxidation by quinoprotein methanol dehydrogenase.
The mechanism of bacterial methanol dehydrogenase involves hydride equivalent transfer from substrate to the ortho-quinone PQQ to provide a C5-reduced intermediate that subsequently rearranges to the hydroquinone PQQH(2). We have studied the PQQ reduction by molecular dynamic (MD) simulations in aqueous solution. Among the five simulated structures, either Asp297 or Glu171 or both are ionized. ...
متن کاملOxidation of Methanol using Carburized Molybdenum Catalyst
Molybdenum catalysts carburized in CH4/H2 exhibited activity for methanol electrooxidation (MOR) in half cell measurements. Mo carburized at 773 and 873 K showed high electrooxidation of methanol compared to carburization at other temperatures. Catalyst characterization was carried out by XRD and TPC. The active carbide species responsible to facilitate the MOR was the Mo oxycarbide and –Mo2C s...
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Dimethoxymethane was synthesized from the direct oxidation of methanol with high conversion and selectivity over specially designed bifunctional V(2)O(5)/TiO2 catalysts with redox and enhanced acidic character, in which the surface acidity played an essential role for inhibiting the formation of formaldehyde through the enhanced condensation reaction of formaldehyde with methanol to produce dim...
متن کاملMethanol Oxidation on Cu(110)
In this paper we highlight the important contribution made by Bob Madix to the understanding of catalysis at single crystal surfaces, focusing here on a favourite model system of his, namely the oxidation of methanol on Cu(110). Madix showed clearly that the methoxy was the pivotal intermediate involved, and it is bound to the surface through the oxygen atom. Here we demonstrate the nature of t...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1988
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)80898-6